Category Archives: Website
You can now use NBLAST to find similar neurons, or GAL4 drivers that label a neuron of interest. The implementation is described here, and the how, with video demos here. You can start NBLASTing right away on the online app!
Neurons listed in the NBLAST results are linked to VFB, so you can see them on the viewer, together with any other image.
And see here how to find GAL4 drivers that label specific neurons.
We are proud to announce the addition of a triple stained adult Brain stack to VFB.
Earlier this year, Kei Ito and colleagues on the BrainName consortium published their long awaited new nomenclature for the insect Brain. VFB has followed the evolving BrainName nomenclature since its inception. We are now fully compliant with the published version and have added the gorgeous triple stained half brain shown in this paper to our hosted stacks. (The raw data for this stack is openly available on FlyBase (details and link here).
The detailed, painted mask on this stack includes major tracts such as the great commissure (blue, centre in the above image). We are working on adding a second mask with many more tracts marked. We are also planning to add linked thumbnails from term info to painted domains on both adult brain stacks.
In other news – usage of VFB continues to climb dramatically. We now get over 18,000 hits a month, with all of our many features getting significant usage.
We’re collecting user stories to assess dev priorities. Please contact us via our blog, or our community group firstname.lastname@example.org to let us know.
Feel free to include anything you’d like to use VFB for, not matter how technically difficult it may seem. Please let us know any frustrations you have with current functionality.
New VFB release: queries available for all neuroanatomy terms and Janelia FlyLight 3D images on stack viewer
We are pleased to announce a new release of VFB, including the ability to query the whole adult, larval and embryonic neuroanatomy, and to layer the Janelia FlyLight 3D images on the stack viewer.
Users can now choose to query ‘All Neuroanatomy’ or to restrict their queries to ‘Adult Brain’. Queries can now be done on , the adult thoracico-abdominal ganglion, sense organs, and motor neurons, among others. For example, searching for ventral cibarial sense organ:
Find the term on the autocomplete and select it.
From here, further queries on expression are possible, just as previously for the adult brain.
We have also added the ability to layer the 3D Janelia FlyLight images on stack viewer. Users can now build composite images with any of the Janelia lines, FlyCircuit neurons, neuroblast and fruitless clones.
Janelia 3D images can be added from any expression queries, clicking on ‘See in Viewer’. For example, a query for transgenes expressed in the ‘wedge’ lists:
Looking at GMR27B03 in the stack viewer:
You can now build composite images with the Janelia lines. For any image in the stack viewer, click ‘Add to composite view’, and then go back to run more queries and add any other images you want (up to 3 at a time for each composite).
You have any any feedback please let us know!
New VFB release – extensive lineage clone data and images added; layer multiple 3D images in the stack viewer
We are excited to announce a new release of VFB, featuring a full set of adult central brain lineage clone images and mapping data from Ito et al., 2013 & Yu et al., 2003 + fruitless expressing clones from Cachero and Ostrovsky et al., 2010. The mapping data we’ve added includes mapping to brain regions and mapping of single neurons and fru expressing lineage clones to complete central brain lineage clones. We’ve also added functionality for display of multiple images on our stack browser. In combination with new functionality allowing multiple registered images. The combination of these functionalities provides opportunities for querying and exploring lineage data that are unavailable from any other resource.
To query by region:
All clones from the Ito and Yu papers and most from Cachero come with linked thumbnail images.
Any of these thumbnails can be added to the stack browser:
You can layer up to 3 image stacks in a single browser view:
Images that can be added to the stack browser include > 16000 neurons from the FlyCircuit dataset. We plan to add downloads for many of our registered stacks in the near future.
Many thanks to Kei Ito & Tzumin Lee for providing us with data and mapping tables to make this release possible.
We hope you enjoy the new content and features. Please let us know what you think,
The VFB team.